Détails Publication
Detection of blaCTX-M, blaTEM, and blaSHV genes in ESBL producing enterobacterales from poultry farms in the peri-urban area of Ouagadougou, Burkina Faso,
Discipline: Sciences biologiques
Auteur(s): Isidore Juste Ouindgueta Bonkoungou, Marguerite Edith Malatala Nikiema, Zakaria Garba, Evariste Bako, Souleymane Belem, Djifahamaï Soma, Fatimata Bintou Josiane Diarra, Barthélémy Sibiry Zoma, Modeste Gampene, Namwin Siourimè Somda, Souleymane Sore and Nicolas Barro
Auteur(s) tagués: BARRO Nicolas
Renseignée par : BONKOUNGOU Isidore Juste Ouindgueta
Résumé

This study investigated the carriage of blaCTX-M, blaTEM and blaSHV gene in Extended-spectrum beta- lactamase (ESBL)-producing Enterobacterals from poultry in peri-urban area of Ouagadougou, Burkina Faso. From June to November 2023, fresh fecal samples were collected from 37 consenting poultry farms in four peri-urban areas around Ouagadougou. These samples were cultured to isolate ESBL- producing Escherichia coli and Klebsiella species. The isolates were then examined for the presence of blaCTX-M, blaTEM, and blaSHV genes and their antibiotic susceptibility patterns. Out of the 37 poultry samples, 27 ESBL-producing bacteria were recovered; 48.64% (18/37) were ESBL-producing E. coli (16 blaCTX-M genes, 8 blaTEM genes, 2 blaSHV genes) and 27.02% (10/37) were Klebsiella spp. (10 blaCTX-M genes, 8 blaSHV genes, 5 blaTEM genes). Co-carriage of blaCTX-M/TEM, blaCTX-M/SHV, and blaTEM/SHV, high resistance rates to tetracycline and trimethoprim-sulfamethoxazole, and multi-drug resistance (MDR) phenotypes were observed among the ESBL-producing bacteria isolated in this study. The findings indicate the widespread presence of ESBL-producing bacteria in poultry farms in Burkina Faso, highlighting a potential public health risk.

Mots-clés

Detection of extended-spectrum beta-lactamase (ESBL), enterobacterales, poultry frams, peri- urban, Burkina Faso

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