The development of bacterial resistance to available antibiotics is a worldwide problem and recent researches
focused on the development of new strategies to combat this bacterial resistance. The current study was
designed to evaluate the inhibitory property of the methanol bark extract from Acacia dudgeoni on cefotaxime
resistant growth of Escherichia coli. The capacity of extract to inhibit the E. coli growth was evaluated in vitro by
using agar diffusion assay. The free radical scavenging property of extract was assessed by using the standard
DPPH assay. Furthermore, flavonoids and phenolic contents of extract were determined spectrophotometrically.
Extract at concentration of 50 µg/mL showed an interesting inhibitory activity on E. coli strain 376 growth that is
resistant to the antibiotic cefotaxime. Moreover, extract exhibited a good DPPH scavenging activity with IC50
value ranged from 16.52 ± 1.04 μg/mL. The flavonoid and phenolic contents of extract were 7.31 ± 0.2 mg EQ/g
of extract and 14.25 ± 1.25 mg EAG / g extract respectively. The methanol bark extract from A. dudgeoni is a
potent source of bioactive compounds to combat bacterial resistance.

Acacia dudgeoni, cefotaximase, Bacterial resistance, Escherichia coli