Uropathogenic Escherichia coli (UPEC) are main cause of Urinary tract infections (UTIs).
UPEC Biofilm-producer have a high level of resistance to antibiotics and this leads to
recurrent episodes of UTIs. This study tested the effects of aqueous garlic extract (AGE) on
biofilm formation and antibiotics susceptibility of multidrug resistant (MDR) UPEC. The
ability for in vitro biofilm formation was detected in 35 MDR UPEC isolates in the absence
and presence of sub-MICs of AGE (15, 30 and 60 mg/ml). The minimum inhibitory
concentrations (MIC) of the AGE were determined by broth microdilution method. The
biofilm formation by strong and moderate biofilm producers was evaluated by measuring
the DO595. we observed high resistance of UPEC (n=35) against the penicillin group,
cephalosporin, Nalidixic acid, fluoroquinolone and tetracycline group In contrast, they were
less resistance to Amikacin (17.15%), Imipenem (11.42%) and Gentamicin (5.71%) but
None of the UPEC was completely sensitive to all the tested antibiotics. UPEC isolates were
susceptible to AGE in the range of 29±2 - 32±4.5 mm and 24±1 - 25±1 mm of Diameter of
zone inhibition respectively at 1000 and 500mg/ml. The MICs values were in the range of
62.5 and 100mg/ml. Sub-MICs AGE inhibited bacteria adhesion at 45.8, 78.8, and 81.1%,
respectively. It also inhibited the biofilm formation and dispersal. The use of AGE in
parenteral preparations to treat UTIs could greatly improve the clinical outcome. There is a
continuous need for the development of new strategies for treatment of UTIs and recurrent
episodes

Extrait d'ail, Biofilm, Antibiotiques, Multirésistance, Escherichia coli, Togo