First report of the L1014S kdr mutation in wild populations of Anopheles gambiae M and S molecular forms in Burkina Faso (West Africa)

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Résumé

We investigated the occurrence of the L1014F and L1014S kdr mutations in malaria vector populations
in Burkina Faso (West Africa). A cross-sectional survey was conducted at 10 sites all located in cotton
cultivation areas which are assumed to be the major insecticide resistance selection foci in Burkina Faso.
The hot ligation method was used to detect the two kdr mutations in field collected Anopheles gambiae
s.l. samples. For the first time in Burkina Faso the L1014S mutation was identified in both M and S forms
of An. gambiae s.s. populations collected from the site of Koupela in the central-eastern region at low
frequency. Furthermore, the L1014S mutation was also found in one specimen of An. arabiensis collected
from the Dano site. The data generated in this study provides additional evidence of the spread of the
L1014S mutation into An. gambiae s.l. populations in West Africa. It is now important to evaluate the role
of the L1014S mutation in the pyrethroid resistance phenotype and assess its potential impact on the
efficacy of pyrethroid-based control measures in West Africa where several resistance mutations now
coexist

Mots-clés

Anopheles gambiae s.l,. L1014F and L1014S, kdr Insecticide resistance, Malaria , Burkina Faso