GC-MS Analysis and Antibacterial Activities of <i>Feretia apodanthera</i> Del. (Rubiaceae) and <i>Ozoroa insignis</i> Del. (Anacardiaceae)
- Journal of Diseases and Medicinal Plants , 5 : 52-52
Résumé
Two medicinal plants, <i>Feretia apodanthera</i> and <i>Ozoroa insignis</i>, used in west African folk medicine to treat infectious diseases, were investigated for their antibacterial potential and their biocidal components. Two extractions were processed in water and aqueous-acetone (70%) and further fractionated by column-chromatography. Both extracts and fractions exhibited selective activity against human pathogenic bacteria as assayed by disc diffusion and microdilution methods. The aqueous-acetone extract of <i>F. apodanthera</i> was active against all the Gram-negative and Gram-positive bacteria (d ≥ 8 mm; MIC ≤ 2.5 mg/ml) while the same extract from <i>O. insignis</i> got markedly activity on Gram-negative bacteria <i>E. coli</i> and <i>K. pneumonia</i> (d ≥ 11 mm). However, the fractions (20µg) of both two plant species were selectively more active on Gram-negative bacteria (d ≥ 11 mm). Tetramethyl silicate, trifluoroamine oxide and neophytadiene were identified by GC-MS as the main volatile compounds present that enhance the antibacterial effects in synergy with others.
Mots-clés
Antibacterial activity, Bacteria, Traditional medicine, Acetone, Chemistry, Broth microdilution, Pathogenic bacteria, Chromatography, Botany, Biology, Minimum inhibitory concentration, Organic chemistry, Medicine