Involvement of Polymorphisms of the Interleukin 17A (rs2275913) and 17F (rs763780) Genes in the Development of Cervical Cancer and HPV Infection in Two West African Countries

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Résumé

Background: Interleukin-17 (IL-17) is a pro-inflammatory cytokine associated with inflammation, autoimmune disorders, and even tumors. Several studies have revealed abnormally high expression of IL-17 in human malignant tumors. The main objective of this study was to analyze the association between Single Nucleotide Polymorphisms (SNPs) of IL-17A (rs 2275913) and IL-17F (rs 763780) and their involvement in the development of cervical cancer in Burkina Faso and Togo, as well as their association with HPV. Methodology: In the present study, two SNPs in IL-17A (rs 2275913) and IL-17F (rs763780) were analyzed in 44 patients with Cervical Cancer (CC) and 93 women with no history of cervical cancer from the city of Ouagadougou and 114 women with no history of cervical cancer from the city of Kara. Nucleic acids were extracted using the R-Biopharm kit. Polymorphism genotyping was performed by real-time PCR using TaqMan® tests. Results: Our results indicated that the G and A alleles were not associated with the different characteristics studied in the two cities. The frequency of genotypes of the rs2275913 polymorphism of the 1L-17A gene was 91.4%, 7.5%, and 1.1% for the GG, GA, and AA genotypes in Ouagadougou, 91.23%, 7.90%, and 3.23% for the TT, TC, and CC genotypes in Ouagadougou, and 89.50%, 10.50% and 0.00% respectively in Lomé in the city of Kara. The mutation rates of rs 2275913 and rs 763780 polymorphisms were 4.84% and 12.90% respectively for Ouagadougou, compared to 4.83% and 5.26% for Kara. There was no statistically significant difference between the genotypes of IL-17A (rs 2275913) and IL-17F (rs763780) polymorphisms in cervical cancer cases, and controls in Ouagadougou. Certain characteristics such as age at first sexual intercourse and parity were associated with IL-17A (rs 2275913) genotypes in Ouagadougou and age at first sexual intercourse in Kara. Conclusion: The results indicate that IL-17A and IL-17F polymorphisms are influenced by certain characteristics of women, but are not involved in the development of cervical cancer.

Mots-clés

Cervical Cancer, HPV, IL-17, Polymorphisms, Burkina Faso, Togo