Screening and molecular identification of indigenous strains of acetic acid bacteria isolated from mango biotopes in Burkina Faso

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Résumé

Acetic acid bacteria (AAB) are useful in industrial production of vinegar. The present study is carried out to
characterize indigenous strains of acetic acid bacteria from mangos in Burkina Faso. Samples of fermented mangos
were used as potential source of acetic acid bacteria. The AAB strains were isolated and identified phenotypically
and using biochemical properties. rRNA 16s gene of Acetobacter genera was used for molecular identification
using conventional PCR technic. Results revealed that fifteen strains were routinely identified as acetic acid bacteria
strains due to their biochemical and physiological properties. Electrophoresis gel of PCR products demonstrated
that the fifteen strains were AAB. Using two primers pairs like 16sF and 16sR, twelve strains among fifteen were
confirming to belonging Acetobacter sp. as CRSBAN- BVA1, CRSBAN- BVA2, CRSBAN- BVA3, CRSBAN-
BVK1, CRSBAN- BVK2, CRSBAN- BVI1, CRSBAN- BVS1, CRSBAN- BVS2, CRSBAN- BVB1, CRSBAN-
BVB2, CRSBAN- BVL2, and CRSBAN- BVP2 which showed each a clear band roughly 1200 Pb. Sequencage
of 16s rRNA showed that bacteria strain CRSBAN- BVK2 has presented 100 % similarity with Acetobacter
tropicalis.The results suggest that fermented juice of mango was a potential biotope source of indigenous acetic bacteria

Mots-clés

Mango fermented juice, Indigenous strains, Acetic acid bacteria, Molecular identification