Aims: In Burkina Faso, the majority of plant-based recipes are a combination of plant organs from
the same or different species. For these recipes’ producers, the aim is to improve efficiency.
However, our ethnobotanical surveys have revealed that for some species, efficacy is better when
the species is not combined, or when it is combined in small proportions with other species. The
aim of this study is to assess the effect of plant organ combination on free radical scavenging
activity, phenolic compound content and flavonoid content.
Study Design: The work combined harvesting and processing of plant material from September to
November 2023, and laboratories analysis from January to March 2024 at Natural Substances
Departement of Institut de Recherche en Sciences Appliquées et Technologie, Ouagadougou.
Methodology: We prepared two batches of recipes, first combining two species from the same
genus, then two species from different genera. The powder of each plant without combination was
also used to compare data. Phytochemical screening was first carried out with TLC and LC-MS
analysis on each plant extract. We then assessed the phenolic compound content (PCC) using
Folin-Ciocalteu method, flavonoid content (FCC) using aluminum chloride test, antioxidant content
using TEAC method and free radical scavenging activity using DPPH method, for different batches.
Results: Phytochemical constituent in the three species are phenolic compounds, terpenoids and
nitrogen derivatives. The results of biological property evaluation suggest that for best efficacy and
to obtain a high phenolic compound content, Lippia multiflora leaves should be used separately, or
combined in 70% proportions with Lippia alba, then in 50% proportions if the combination is made
with Ocimum basilicum.
Conclusion: This research has provided relevant results to guide players in the field of traditional
medicine and users of herbal remedies.

Phytochemistry; free-radical properties; plants organ combination; herbal recipes