Lannea acida is a medicinal plant used in treatments for diabetes and infertility.
This study’s purpose was to evaluate the safety of Lannea acida trunk bark. Phytochemical
screening was performed on the hydroethanolic extract of Lannea acida trunk bark. Acute
innocuity was assessed in female NMRI mice with a limited dose of 2000 mg/kg bw (body
weight). Subacute safety was investigated too in female Wistar rats throughout 28 days by
daily administration. Four groups of six rats were formed and received distilled water and the
extract at 40, 100, and 200 mg/kg bw respectively. Two other groups served as satellites and
received water and a higher dose. Phytochemical screening revealed the presence of tannins,
polyphenols, coumarins, alkaloids, triterpenes, and steroids. The quantitative assay revealed
polyphenols, flavonoids, and tannins levels 649.77±0.36 mg EAG/g, 7.46±0.13 mg EQ/g, 79.71
± 2.78 mg EAT/g, respectively. The extract administered as a single dose did not cause any
mortality. The LD50 is greater than 2000 mg/kg. At repeated doses for 28 days, the extract did
not show any significant (p>0.05) variation in animal weight. No dose of extract caused a
significant (p>0.05) variation in relative organ weights. Blood counts showed no significant
(p>0.05) variation. The following biochemical parameters did not vary significantly (p>0.05):
Urea, creatinine, total bilirubin and direct bilirubin, triglycerides, total cholesterol, AST, and
ALT. The liver functions of the rats were not affected. This study shows that the hydroethanolic
extract of Lannea acida trunk bark is not toxic for the single and medium term.

Acute Toxicity; Hydroethanolic Extract; Lannea Acida, LD50; Subacute Toxicity