Publications récentes
Article
Optimization of Antigen Concentrations to Improve the Signal to Noise Ratio of ELISA Tests for the Detection of Anti Plasmodium falciparum Antibodies
Nicolas OUEDRAOGO, Fatimata THIOMBIANO, Oumarou OUEDRAOGO, Mireille OUEDRAOGO, Guillaume Sylvestre SANOU, Harouna SORE, Casimire Wendlamita TARAMA, François TAPSOBA, Yéri Esther HIEN, Kaba Mariama CHERIF and Aly SAVADOGOMalaria, caused by the parasite Plasmodium falciparum, remains a major public health priority in tropical regions. The Enzyme-Linked Immunosorbent Assay (ELISA) for detecting anti-Plasmodium antibodies is an essential diagnostic tool. However, its sensitivity and specificity are closely dependent on the concentration of antigens used.
This study aimed to determine the optimal antigen concentration for seven P. falciparum antigens (AMA1, MSP1, GLURP, CSP, EBA175, MSP3, and EXP1) in order to optimize the signal-to-background noise ratio of indirect ELISA tests. To achieve this, titration microplates were coated with serial dilutions of each antigen (from 2 μg/ml to 0.0625 μg/ml), incubated with patient sera, and then developed using conjugated secondary antibodies. Each antigen concentration was tested in duplicate wells (n=2), using Hyper-Immune Tanzanian (HIT) plasma at antigen-specific...
Article
Functional, physicochemical, and microbiological properties of Djir, a traditional fermented pearl millet flour from Chad
Moussa Idriss Mahamat, Edwige Rosine Somé/Tiodjio, Kagambèga Boureima, Cissé Hama, Mahamat Bechir, Abdelsalam Tidjani, Donatien Kaboré and Aly SavadogoCe travail de recherche a permis de caractériser pour la première fois les propriétés fonctionnelles, physico-chimiques et microbiologiques du Djir, une farine fermentée traditionnelle à base de mil perlé (Pennisetum glaucum) largement consommée au Tchad. Quatre-vingt-dix échantillons ont été collectés dans trois villes tchadiennes (Abéché, Guéréda et Oum-Hadjer), aussi bien sur des sites de production que sur des marchés. Les résultats ont montré que le Djir présente un pH acide (3,39 à 3,93) et une faible teneur en humidité (2,50 à 10,68 %), deux facteurs favorables à sa conservation. Les propriétés fonctionnelles varient considérablement d’un échantillon à l’autre, avec des capacités d’absorption d’eau comprises entre 68 et 128 %, des capacités d’absorption d’huile entre 60 et 109 %, et une stabilité de la mousse particulièrement élevée (88 à 99 %). L’analyse microbiologique a révélé...
Article
Analysis of Water Mass Flow Rate Influence on a Crystalline Silicon Cell Temperature and Electronic Parameters under Concentrated Light
Mahamadi Savadogoa, Boubacar Soro, Mamoudou Saria, Abdoulaye Sawadogo, Abdul-Aziz Zongo, Issa Zerbo, Martial ZoungranaThis study aims to analyze the effect of water mass flow rate on a Si-solar cell temperature and electronic parameters through two models. From the thermal model, the thermal equation is determined and solved to obtain the cell temperature versus light concentration and for different mass flow rates. By the electrical model, the electronic parameters were determined and plotted also versus light concentration and water mass flow rate. Results show that temperature rises with light concentration and decreases with increasing mass flow rate. Mobility and diffusion coefficients of electrons and holes as well as the silicon gap energy, decrease with light concentration but all increase with rising mass flow rate. The electrons intrinsic density increases with concentration and fall down with mass flow rise. Near the...
Article
Influence of anthropization on floristic diversity and carbon stocks in a Sudano-Sahelian vegetation landscape
Guiatin, E., Zoungrana, A., Bazié, H.R. et Lompo D.J.PMosaic landscapes integrating protected areas and agroecosystems with variable exploitation intensities constitute critical ecosystems for biodiversity conservation and ecosystem service provision. This study evaluated how anthropogenic pressure gradients influence plant community structure, diversity, and carbon sequestration in the Sudano-Sahelian zone of Burkina Faso. Using a stratified sampling design across four anthropization levels: (i) savannah woodland = level 1, (ii) forest-edge fields = level 2, (iii) bush fields = level 3, and (iv) homestead fields = level 4, we analyzed 80 forest plots distributed along transects from inhabited areas toward the Sissili classified forest. Within each plot, floristic composition, dendrometric parameters, and aboveground carbon stocks were measured. The results revealed a significant decline in woody species richness from savannah woodland (21 ...
Article
A Novel Reference Model for Intelligent and Comfortable Longitudinal Vehicle Control: Theory, Optimization, and Validation
Flavien H. Somda, Désiré Guel, Kisito K. Kaboré, Antoine SchorgenThis paper introduces a novel reference model for intelligent longitudinal vehicle control, designed to enhance both safety and passenger comfort. The proposed model dynamically adjusts the follower vehicle’s acceleration based on its penetration distance relative to the lead vehicle, ensuring smooth speed transitions and adaptive deceleration. By preventing abrupt braking, the model maintains a safe inter-vehicle distance while reducing passenger discomfort. Key contributions include an analytical derivation of the follower vehicle’s dynamics and a novel formulation of the safety distance using the Lambert W function, enabling precise parameter optimization. A dedicated optimization framework ensures compliance with safety constraints while minimizing excessive acceleration and jerk. The model’s performance is validated through numerical simulations in various driving scenarios, includi...
Article
Prevalence of Anemia and Its Associated Factors Among Public Primary School Children in the Plateau Central Region of Burkina Faso
Daniel Somwaoga Ouedraogo, Ella W. R. Compaore, Ousmane Ouedraogo, Mamoudou H. DickoAnemia is a public health problem afecting mainly children under 5 years and pregnant women. However, school-age children are also afected but are often neglected in research. This study aimed to assess the prevalence and factors associated with anemia among primary school children in public schools in the Plateau Central Region of Burkina Faso. Hemoglobin (Hb) concentration was determined using a HemoCue HB201þ analyzer. Data were collected from 551 schoolchildren aged 5–14 years. Anemia was established if the Hb level was lower than 11.5 g/dL in children aged 5–11 years and less than 12 g/dL in children aged 12–14 years.
Binary logistic regression was adjusted to identify factors associated with anemia at a p value < 0.05. The prevalence of anemia was 59.2%, including 52.6% of the girls. Schoolchildren aged 5–11 years were the most afected, with a rate of 58.5%, versus 60.2% for schoo...
Article
Study of Ageratum conyzoides L. (Asteraceae) Phytochemical Composition as Quorum-sensing Inhibitors to Combat Antibiotic-resistant Bacterial Infections
Eli Compaoré, Lassané Ouedraogo, Ablassé Rouamba, Vincent Ouedraogo, Alimata Bancé, René Dofini Magnini, Safiétou Ouedraogo, Yasmine Aminata Bangré, Moussa Compaoré and Martin KiendrebeogoThe rise of antibiotic-resistant bacterial infections has intensified the search for plant-derived quorum-sensing (QS) inhibitors as alternative therapeutic strategies. Ageratum conyzoides L. has a long history of traditional use as an antimicrobial agent against multidrug-resistant pathogens, yet the phytochemical basis of its QS-inhibitory activity remains incompletely characterized. This study aimed to investigate the phytochemical composition of A. conyzoides and evaluate its potential as a QS inhibitor and antioxidant. Bioassay-guided fractionation of the methanol extract was conducted using flash chromatography on a C18 column. High-performance thin-layer chromatography (HPTLC) was used to establish the phytochemical profile of active subfractions. Antioxidant activity was assessed using the DPPH radical scavenging, ferric reducing antioxidant power (FRAP), and ABTS Trolox equivale...
Article
Défis contemporains d'une souveraineté nationale: lecture croisée de quelques romans burkinabè
Pascal KOMBOIGO et Lamoussa TIAHOLe roman burkinabè pose littéralement l’épineuse question de la recherche des voies et moyens en vue d’un réel progrès sur le continent africain en général et pour le Burkina Faso en particulier. Pour ce faire il ressort de la conscience des œuvres qui s’ouvrent à notre analyse que la dominée société textuelle romanesque est marquée par de nombreuses contre valeurs socioculturelles. Ces contre-valeurs censées être combattues par un système viable ne le sont pas car la volonté de certains actants à promouvoir les valeurs sociales et culturelles constructives se heurte malheureusement à un désordre systémique qui nourrit et entretient les contre valeurs socioculturelles. Nous nous intéressons ici à la vision du monde des auteurs dans leurs romans, de relever les défis auxquels sont confrontés les États par le biais du structuralisme et de la narratologie.
Article
Données techniques, chronologiques et historiques de la sidérurgie directe dans le Gulmu
THIOMBIANO Foniyama Elise épouse ILBOUDOLa province du Gulmu est l’une des localités ayant bénéficié raisonnablement
d’investigations scientifiques sur la question de la production ancienne du fer (J.B.
Kiethéga, 2009 ; F.E. Thiombiano/Ilboudo, 1991, 2010 ; O. Yougbaré, 1992 ; H.
Lankoandé, 2015, 2018, 2023). Les résultats des travaux montrent un potentiel
archéométallurgique conséquent. De même, l’analyse des vestiges révèle, au stade
actuel des recherches, deux catégories de fourneaux selon le mode opératoire,
soutenues par une variabilité de traditions techniques. Les datations radiocarbones
réalisées sur les fourneaux de la région mettent en évidence des séquences
chronologiques qui s’étalent du VIe au début du XXe siècle de notre ère, permettant
non seulement d’apprécier l’évolution temporelle de cette activité ancestrale mais
aussi d’analyser la dynamique du peuplement de la région étudiée.
Pour l’...
Article
Assessment of Physicochemical Characteristics and Microbiological Quality of Fried Shrimps Sold in Rural Municipality of Loumbila
Cissé Hama, Nebié Sibiri, Zongo Oumarou, Traoré Roukiatou, Djibrine Adoum Oumar, Konwougoum Inoussa, Zingué B. A. Romaric, Tapsoba François, Sawadogo Adama, Abdelsalam Tidjani and Savadogo AlyShrimp is a crustacean found in marine and freshwater environments and is widely distributed throughout the world due to its nutritional and socioeconomic values. However, its chemical composition, rich in proteins and lipids, and its water content, make it an easily perishable food commodity and particularly susceptible to microbiological and physicochemical alterations. As part of the study, ten samples of fried shrimps were collected from different sales sites in Loumbila. Physicochemical characteristics and microbiological quality were assessed using standard analytical methods. TMAF loadings ranged from 0.29±0.12x106 CFU/g to 1.83±1.29x106 CFU/g. Dry matter and water content ranged from 87.23±1.25 % to 95.92±0.67 % and 4.08±0.67 % to 12.77±1.25 %, respectively. Also, acidity and pH ranged from 0.04 % to 0.12±0.01 % and 8.48±0.06 and 8.99±0.11, respectively. As for the loads of therm...