Publications récentes
Article
Phylogenetic Groups and Antibiotic Resistance Genes of Escherichia coli isolated from Food and Humans in Ouagadougou, Burkina Faso
Dissinviel Stéphane Kpoda, Namwin Siourimè Somda, Adama Patrice Soubeiga. Moustapha Soungalo Drabo, Oukanou Tankoano, Mohamed Baguy Ouattara, Paulette Karfo, Fulbert Nikiema, Elie Kabré, Nathalie Guessennd, Solange Kakou-ngazoa, Sidiki Aboubakar OuattaraEscherichia coli is a major inhabitant of the intestinal tract of most mammalian species, including humans and animals.
This bacterium is classified into four main phylogenetic groups (A, B1, B2, D), with pathogenic strains primarily belong
ing to group B2 and, to a lesser extent, group D. This study investigated the correlation between E. coli phylogenetic
groups and antibiotic resistance by analyzing 144 strains (95 clinical, 49 food-derived). Species identification was con
firmed via uidA gene PCR, and antimicrobial susceptibility was assessed using the Kirby-Bauer disc diffusion method.
Extended-spectrum beta-lactamase (ESBL) production was determined through the double synergy test, and phylogenetic
classification was performed using triplex PCR. Clinical isolates exhibited high resistance to cephalosporins (up to 56%)
and fluoroquinolones (57%), whereas food-derived str...
Article
Effects of Heating Time and Extraction Method on the Antioxidant Properties of Teas Consumed in Burkina Faso
Ghislain Téwendé KOBENDE, Samson GUENNE, Arthur A. A. DABONE, Modeste TRAORE, Hemayoro SAMA, Mamounata DIAO, Adama HILOU, Martin KIENDREBÉOGO and Mamoudou Hama DICKOTea preparation conditions can influence the recovery of bioactive compounds and the antioxidant properties of the resulting beverage. This study compared the effects of heating time and extraction procedure on ten commercial tea samples consumed in Burkina Faso. Each sample underwent two successive decoctions at 100 °C for either 30 min or 1 h and three successive hydro-methanolic macerations. The extracts were examined through qualitative phytochemical screening, quantitative determination of total phenolics, total flavonoids and theophylline, and antioxidant assessment using DPPH, FRAP and SOD assays. Sterols and triterpenes, flavonoid aglycones, tannins and saponins were detected in all extracts. Simida tea showed the highest reported total phenolic content in the decoction extracts (29.42 ± 1.04 mg GAE/100 mg), whereas Moringa tea showed the highest total flavonoid content (5.581 ±...
Article
Formalizing PI Planning in Scaled Agile with Model-Driven Engineering: A Metamodel and Framework for Strategic Alignment
Flavien Somda ; Désiré Guel ; Kisito KaboreProgram Increment (PI) Planning is a critical synchronization event in scaled Agile methodologies, yet remains largely informal, with limited analytical rigor or strategic alignment. This paper introduces a formal metamodel and a model-driven framework to support structured PI Planning in large-scale software development. Our metamodel captures essential planning artifacts, including features, teams, iterations, risks, and dependencies, and is enriched with OCL constraints for validation and traceability. We extend the model to align planning with business value streams and introduce stakeholder-specific viewpoints for tailored analysis. Compared to tools like Jira or Mural, our approach provides formal semantics, automated reasoning, and extensibility. This work lays the foundation for a Domain-Specific Language (DSL) for PI Planning, bridging the gap between Agile practice and formal m...
Article
Phenotypic Evaluation and Genetic Parameter Estimation of a Collection of Bambara Groundnut [Vigna subterranea (L.)] Landraces in the Sudan–Sahelian Zone in Burkina Faso
Mariam Kiebre, Jacques Ouedraogo, Ignace Tondé, Jocelyne Wendpingrenoma Ouangraoua, Clémence Zerbo, Zakaria Kiebre, Romaric Kiswendsida Nanema, Fanta Reine Sheirita Tietiambou, Hamid El Bilali, Filippo Acasto and Jacques NanemaEffective utilization of plant genetic resources requires agro-morphological evaluation of collections across diverse growing regions. The objectives of this study are to assess genetic diversity and estimate genetic parameters through morphological traits of Bambara groundnut. The study was conducted over two growing seasons at the SUSTLIVES project site in Gampela (Central Burkina Faso) using an RCBD with three replicates. A total of eight qualitative and eight quantitative traits were used to estimate variability within a collection of 153 landraces. The results revealed a substantial variability in all the qualitative traits, with the number of modalities ranging from 2 to 10, seed color being the variable trait. Based on qualitative traits related to seed, the landraces were grouped into 12 morpho-groups. The analysis of variance showed the presence of significant variation between...
Article
Detection of Genetic Supports Regulated the Quorum Sensing in Multidrug-Resistant P. aeruginosa and E. coli
Comoé Koffi Donatien BENIE, N’zebo Désiré KOUAME, Amin Paulin YAPI, Adjaratou TRAORE, Koua ATOBLA, Bonny Aya Carole, KPODA Dissinviel Stéphane, Nathalie GUESSENND , Adjéhi DADIE.Quorum sensing (QS) is a system of intercellular communication and regulation of the
transcription of resistance, virulence, and pathogenicity genes. The aim of this study was to
identify genetic markers controlling quorum sensing in multidrug-resistant P. aeruginosa and E.
coli. A set of fifty (50) strains, composed of P. aeruginosa (30) and E. coli (20), were isolated
from animal products. These strains underwent phenotypic and biochemical identification.
Antibiotic resistance profiles were determined by the Muller-Hinton agar diffusion method.
Quorum sensing markers (LasI/LasR) and (RhlI/RhlR) were detected by PCR. The prevalence of
the Las gene (LasI/LasR) and the Rhl gene (RhlI/RhlR) was 80% and 60%, respectively, in P.
aeruginosa. In E. coli, the prevalence of QS genes was 40% for Las (LasI/LasR) and 40% for
Rhl (RhlI/RhlR). The total prevalence of the Las gene an...
Article
Response of the Benga tigréVariety of Mung Bean(Vigna radiataL. Wilczek) to the Effects of Mineral Nutrition and Plant Density on the Yield and Yield Components in Burkina Faso
Sidibé Hamadou, Batieno Teyiouê Benoît Joseph, Sawadogo Zakaridja , Sakande Boureima, Niampa Moussa and Sawadogo MahamadouMung bean(Vigna radiata), introduced into Burkina Faso in the 1980s, remains underused in rural production systems. This study evaluated the response of the Benga tigrévariety to fertilisation and sowing density to identify a suitable management combination under the conditions of the Saria research station. The experiment used a randomised complete block design with three replications and combined five fertilisationtreatments—NPK (100 kg/ha), station compost (2,500 kg/ha), Jatropha compost (2,500 kg/ha), station compost plus NPK, and Jatropha compost plus NPK—with six sowing densities. Emergence rate, days to 50% flowering, days to 50% maturity, 1,000-grain weight, pod yield, and grain yield were assessed. Data were analysed using GenStat 12th Edition, and treatment means were compared using the Bonferroni test at the 5% le...
Article
Soil constraints limit the effectiveness of phosphate-solubilizing bacteria in acidic Lixisols of Burkina Faso
Papa S. Sarr , Adama Sagnon , Samson Guenné , Ezechiel B. Tibiri , Saïdou Zongo , Estel P. Name , Seydou Sawadogo , Mamoudou Traore , Nicolas Barro , Satoshi Nakamura & Fidèle TiendrebeogoPhosphorus (P) deficiency limits crop productivity in weathered tropical soils of sub-Saharan Africa, particularly in Lixisols where P availability is low. Phosphate-solubilizing microorganisms may improve P use efficiency and support sustainable crop production. This study evaluated the phosphate-solubilizing and plant growth-promoting potential of microbial strains under Lixisol conditions. Six distinct strains with contrasting taxonomy and P-cycling traits were assessed for inorganic P solubilization and organic P mineralization in vitro using tricalcium phosphate, sodium phytate, and Burkina Faso phosphate rock (BPR). Their ability to mobilize soil P was tested in a pot experiment with cowpea grown in two contrasting Lixisols amended with BPR. In vitro P solubilization was associated with acidification and the presence of phosphate-cycling genes (gcd, pqqE, aphA, phnX). However, this...
Article
The enduring burden of short birth intervals on child survival: A 28-year trend analysis in Burkina Faso
Hervé BassingaChild survival has improved considerably over the past decades in sub-Saharan Africa, yet preventable risk factors such as closely spaced births continue to threaten progress, especially in fragile contexts. This study examines the association between interbirth intervals and under-five survival in Burkina Faso using data from five rounds of Demographic and Health Surveys conducted between 1993 and 2021. The study combines descriptive survival analysis (e.g., Kaplan–Meier curves, log-rank tests) with Cox proportional hazards models to estimate the adjusted association between interbirth intervals and under-five mortality, while controlling for maternal, child, household, and contextual characteristics. The results show that short interbirth intervals (<24 months) are consistently associated with excess mortality, following a markedly non-linear trajectory: the adjusted hazard ratio decli...
Article
Phytochemical Profile and in vitro Antiplasmodial Activity of Ximenia americana L. (Olacaceae) and Waltheria indica L. (Sterculiaceae)
Vincent Rouamba, Alphonsine Ramde-Tiendrebeogo, Nabere Ouattara, Harouna Sore, Ibrahim Ouedraogo, Moussa Compaore, Martin Kiendrebeogo and Innocent Pierre GuissouXimenia americana and Waltheria indica are used in traditional medicine in Burkina Faso for treating malaria in children aged 0–5 years. This study evaluated their phytochemical profiles, antioxidant properties, mineral contents, and in vitro antiplasmodial activities. Leaf and bark extracts of X. americana and whole-plant extracts of W. indica were prepared using solvents of increasing polarity. Phenolic and terpenoid constituents were screened by high-performance thin-layer chromatography, while total phenolic, flavonoid, and terpenoid contents were quantified using colourimetric methods. Mineral contents were determined by inductively coupled plasma atomic emission spectrometry. Antioxidant activity was assessed by DPPH radical-scavenging and ferric-ion-reduction assays. Antiplasmodial activity was evaluated against the chloroquine-sensitive 3D7 and multidrug-resistant Dd2 strains of...
Article
Security constraints, logistical challenges, and coverage of NGO food and nutrition assistance during the 2021–2023 security crisis in Yatenga Province, Burkina Faso
DIALLO Abdoulaye, ZONGO Martine,BAKYONO/NABALOUM Rasmata, ZOMA VincentThe security crisis affecting Burkina Faso since 2015 has considerably complicated humanitarian organizations’ access to vulnerable populations, particularly in the rural areas of Yatenga province in the north of the country. Mobilizing the literature on humanitarian access negotiation and the political economy of aid delivery in conflict settings, this study analyzes the security constraints and logistical challenges faced by fifteen non-governmental organizations (NGOs) in implementing food and nutrition assistance interventions, and their consequences for the coverage rate of assistance provided to internally displaced households. A mixed-methods approach was used, combining a documentary review, semi-structured interviews with program managers, and a structured survey of 200 displaced household heads, conducted between October and November 2023 in Ouahigouya. The results indicate tha...